Gene name |
SPAC22G7.09c |
Gene ID |
24/H07 |
Gene synonyms/obsolete |
|
Gene product |
nucleoporin; nuclear
pore complex; involved in nuclear export of the small
ribosomal subunit |
Entry clone |
Cloned |
ORF length (unspliced) |
1538 |
ORF length (spliced) |
1278 |
Entry clone length |
1538 |
No. of intron |
5 |
Sequence status |
Finished |
Sequence results |
140A:G / 1526C:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC22G7.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTCGGGTTAAATAAAAC |
Rev primer name |
SPAC22G7.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACTTATAAAGGGCAAGGAC |
Amino acid length |
425 |
Molecular weight |
44.9 |
Isoelectric point (calc.) |
4.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSSVSTALLI |
Localization (YFP) |
nuclear envelope;
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |