Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC4A8.01
Gene ID 25/A03
Gene synonyms/obsolete mde5; meu30; SPAC25H1.09
Gene product alpha-amylase; meiotic expression upregulated; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1542
ORF length (spliced)
Entry clone length 1542
No. of intron 0
Sequence status Finished
Sequence results 1098T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC4A8.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGCACAACGAAGTTTT
Rev primer name SPAC4A8.01.Rv
Rev primer SEQ AGAAAGCTGGGTACTTCCTATCGGGCCCATTA
Amino acid length 513
Molecular weight 58.7
Isoelectric point (calc.) 4.9
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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