| Gene name |
SPAC328.03 |
| Gene ID |
25/A05 |
| Gene synonyms/obsolete |
tps1 |
| Gene product |
alpha,alpha-trehalose-phosphate synthase
[UDP-forming]; glycosyl transferase family 20; involved in
trehalose metabolism; involved in stress response (required);
complexed with Ntp1p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1542 |
| ORF length (spliced) |
|
| Entry clone length |
1542 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC328.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGGATGCTCATGATAC |
| Rev primer name |
SPAC328.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACGAGCTAGAATTCATCGAC |
| Amino acid length |
513 |
| Molecular weight |
58.4 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIVVSNRLPI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus, partially |
| Microscope used for
observation |
DeltaVision |