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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC9E9.10c
Gene ID 25/A09
Gene synonyms/obsolete cbh1; cbh
Gene product centromere k-type repeat binding protein; CENP-B homolog; involved in chromosome segregation; CENP-B box; C-terminal dimerization domain; disruption of CENP-B homologs causes a decrease in heterochromatin-specific modifications of histone H3; Sp CENP-B homologs are functionally redundant at centromeres; CENP-B homologs act as site-specific nucleation factors for the formation of centromeric heterochromatin by heterochromatin-specific modifications of histone tails
Entry clone Cloned
ORF length (unspliced) 1545
ORF length (spliced)
Entry clone length 1545
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC9E9.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTCCTATACGCCAGGC
Rev primer name SPAC9E9.10.Rv
Rev primer SEQ AGAAAGCTGGGTACGCTATTAAATTATTAGGG
Amino acid length 514
Molecular weight 59.8
Isoelectric point (calc.) 7.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) SPB; nuclear dots; spindle microtubules
Comments for localization 1~2 dots/nucleus
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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