Gene name |
SPAC57A7.08 |
Gene ID |
25/B01 |
Gene synonyms/obsolete |
phz1; pzh1 |
Gene product |
serine/threonine
protein phosphatase pp-z; involved in salt tolerance; involved
in the regulation of the plasma membrane H+-ATPase; regulates
Na+ ion influx independently of Trk1p |
Entry clone |
Cloned |
ORF length (unspliced) |
1548 |
ORF length (spliced) |
|
Entry clone length |
1548 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC57A7.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGACAGGGGAGCAGTAA |
Rev primer name |
SPAC57A7.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACACGCTTTGGGTAACCTGC |
Amino acid length |
515 |
Molecular weight |
57.1 |
Isoelectric point (calc.) |
6.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LETILLLFL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |