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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC688.14
Gene ID 25/B03
Gene synonyms/obsolete
Gene product transcriptional regulator; SET domain; histone lysine methyltransferase
Entry clone Cloned
ORF length (unspliced) 1551
ORF length (spliced) 1386
Entry clone length 1551
No. of intron 3
Sequence status Finished
Sequence results 155A:G / 689A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC688.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTAAAATGGGCAATCGA
Rev primer name SPAC688.14.Rv
Rev primer SEQ AGAAAGCTGGGTAGTGATCAACCTTTTGCTTT
Amino acid length 461
Molecular weight 53.2
Isoelectric point (calc.) 4.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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