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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2E1P3.02c
Gene ID 25/B09
Gene synonyms/obsolete
Gene product ammonium transporter
Entry clone Cloned
ORF length (unspliced) 1554
ORF length (spliced)
Entry clone length 1554
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC2E1P3.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTTAACGAGCCGAATGC
Rev primer name SPAC2E1P3.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAAACTCATCCTGCTTTTCC
Amino acid length 517
Molecular weight 56.4
Isoelectric point (calc.) 5.3
Signal SEQ
No. of transmembrane domain 11
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLVDLVPGLHI
Localization (YFP) vacuole
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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