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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC227.07c
Gene ID 25/B10
Gene synonyms/obsolete pab1
Gene product protein phosphatase pp2a regulatory subunit b; involved in cellular morphogenesis; involved in cell wall biosynthesis; involved in cytokinesis; WD repeat protein
Entry clone Cloned
ORF length (unspliced) 1556
ORF length (spliced) 1392
Entry clone length 1556
No. of intron 3
Sequence status Finished
Sequence results 472G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC227.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATGATATAGAAGACTC
Rev primer name SPAC227.07.Rv
Rev primer SEQ AGAAAGCTGGGTAGAGCTTAGAGAAAACAAAA
Amino acid length 463
Molecular weight 52.7
Isoelectric point (calc.) 5.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LRINLWNLSI
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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