| Gene name |
SPBC685.02 |
| Gene ID |
25/C04 |
| Gene synonyms/obsolete |
SPBC685.02 |
| Gene product |
conserved
hypothetical; conserved protein; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1564 |
| ORF length (spliced) |
1230 |
| Entry clone length |
1564 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
556T:C / 1355A:G /
1476A:G / 1513A:G / 1557G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC685.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAGAATATGAAGATTT |
| Rev primer name |
SPBC685.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACGGACTTGAAAGAGGGTAA |
| Amino acid length |
409 |
| Molecular weight |
48.3 |
| Isoelectric point (calc.) |
7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
cytoplasmic
dots=mitochondrion? |
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol; cytoplasmic dots |
| Microscope used for
observation |
DeltaVision |