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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC553.01c
Gene ID 25/C07
Gene synonyms/obsolete SPCC736.01c
Gene product conserved hypothetical; hypothetical protein; possibly involved in signal transduction, as the only detectable defect of the null mutant of the S. cerevisiae homolog YGR042w is in heterologous signal transduction
Entry clone Cloned
ORF length (unspliced) 2148
ORF length (spliced)
Entry clone length 2148
No. of intron 0
Sequence status Finished
Sequence results 1672T:C / 1807T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC553.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATACAAGTTCCAATGT
Rev primer name SPCC553.01.Rv
Rev primer SEQ AGAAAGCTGGGTAAATAAAGTCACCATCTTCG
Amino acid length 715
Molecular weight 78
Isoelectric point (calc.) 9.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) SPB; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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