Gene name |
SPAC222.12c |
Gene ID |
25/D07 |
Gene synonyms/obsolete |
atp2 |
Gene product |
F1-ATPase (beta
subunit); ATP synthesis coupled proton transport; functionally
complemented by Sc ATP2 |
Entry clone |
Cloned |
ORF length (unspliced) |
1578 |
ORF length (spliced) |
|
Entry clone length |
1578 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
280T:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC222.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTAAAGAAACAGGCCCT |
Rev primer name |
SPAC222.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGCAGCCAATTCTTGTGCA |
Amino acid length |
525 |
Molecular weight |
56.8 |
Isoelectric point (calc.) |
5.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSRSISELGI |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |