| Gene name |
SPAC16E8.11c |
| Gene ID |
25/D09 |
| Gene synonyms/obsolete |
|
| Gene product |
transcription factor
TFIIH complex; general RNA polymerase II transcription factor
activity; BSD domain (2) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1579 |
| ORF length (spliced) |
1434 |
| Entry clone length |
1579 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC16E8.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTCGTGTTTTTATAGT |
| Rev primer name |
SPAC16E8.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCAGGAGGATAAGACATT |
| Amino acid length |
477 |
| Molecular weight |
54.5 |
| Isoelectric point (calc.) |
5.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol; a few
cytoplasmic dots |
| Comments for localization |
moving dots |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |