| Gene name |
SPAC23H3.14 |
| Gene ID |
25/E03 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; hypothetical protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1584 |
| ORF length (spliced) |
1410 |
| Entry clone length |
1584 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
205A:G / 1342A:T /
1564T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC23H3.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGAAAATGGTGCAAG |
| Rev primer name |
SPAC23H3.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACAGCAGGTTCTTTAGAA |
| Amino acid length |
469 |
| Molecular weight |
53.1 |
| Isoelectric point (calc.) |
5.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVLLKVLLL |
| Localization (YFP) |
site of septum
formation; cytoplasmic dots, especially at cell tip |
| Comments for localization |
aggregates at cell tip
and site of septum formation by over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |