Gene name |
SPAC26A3.01 |
Gene ID |
25/F10 |
Gene synonyms/obsolete |
sxa1;
SPAC2E1P5.06 |
Gene product |
aspartic protease;
involved in conjugation; GPI anchored protein(pers. comm.
Birgit Eisenhaber); glycoprotein; no apparent Sc
ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1602 |
ORF length (spliced) |
|
Entry clone length |
1602 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC26A3.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGGCTTCTTTCTTTGT |
Rev primer name |
SPAC26A3.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCGAAAAGTAAAGAGATC |
Amino acid length |
533 |
Molecular weight |
56.8 |
Isoelectric point (calc.) |
4 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |