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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC13G6.08
Gene ID 25/G06
Gene synonyms/obsolete
Gene product conserved hypothetical; WD repeat protein; Cdc20/Fizzy family; similar to Sp SPCC1620.04C; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1608
ORF length (spliced)
Entry clone length 1608
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC13G6.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTACACTCTACCAGGT
Rev primer name SPAC13G6.08.Rv
Rev primer SEQ AGAAAGCTGGGTAACGGAGATGAGAATCTATT
Amino acid length 535
Molecular weight 60.5
Isoelectric point (calc.) 6.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ambiguous structure
Comments for localization ambiguous structure near nucleus
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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