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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC16C9.04c
Gene ID 25/G07
Gene synonyms/obsolete
Gene product basal transcriptional regulator; zinc finger protein; zf-CCCH type; CCR4-Not complex; rrm RNA recognition motif; RNA-binding protein
Entry clone Cloned#
ORF length (unspliced) 1608
ORF length (spliced) 1470
Entry clone length 1608
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC16C9.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTAAAAACGCAAGAGAT
Rev primer name SPAC16C9.04.Rv
Rev primer SEQ AGAAAGCTGGGTAACTTTGGAAACCAGGAGGA
Amino acid length 489
Molecular weight 54.4
Isoelectric point (calc.) 8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 88
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization changed to: cytosol=nucleus
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images
LMB 2 images) See all images

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