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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1235.13
Gene ID 25/G09
Gene synonyms/obsolete ght6; meu12
Gene product meiotic expression upregulated; hexose transporter; similar to Sp GHT1 and GHT2 and GHT4 and GHT5 and GHT3 and SPCC548.06C and SPBC1348.14C; tandem duplication
Entry clone Cloned
ORF length (unspliced) 1608
ORF length (spliced)
Entry clone length 1608
No. of intron 0
Sequence status Finished
Sequence results 352A:G / 1125T:C / 1158T:C / 1230T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1235.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCGAAAATCTTAACCAT
Rev primer name SPCC1235.13.Rv
Rev primer SEQ AGAAAGCTGGGTATAAGCCAACCGGATTGGTG
Amino acid length 535
Molecular weight 59.4
Isoelectric point (calc.) 8.4
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 10
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LMFFGMLFL/LNSPFLAALIL/LEEINQLYL
Localization (YFP) periphery at cell tip and site of septum formation
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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