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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17A5.04c
Gene ID 25/G12
Gene synonyms/obsolete
Gene product zinc metallopeptidase; disintegrin/ADAM protease; GPI anchored protein (pers. comm. Birgit Eisenhaber); glycoprotein; non-essential; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1610
ORF length (spliced) 1539
Entry clone length 1610
No. of intron 1
Sequence status Finished
Sequence results 1329T:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17A5.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGGCTCGTTTTACTGTT
Rev primer name SPAC17A5.04.Rv
Rev primer SEQ AGAAAGCTGGGTACCAGAAAAACCATGCTATT
Amino acid length 512
Molecular weight 56.4
Isoelectric point (calc.) 5.3
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFPALQTLWI
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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