| Gene name |
SPAC17G6.08 |
| Gene ID |
25/H01 |
| Gene synonyms/obsolete |
pep7; vac1 |
| Gene product |
zinc finger protein;
zf-C2H2 type; zf-FYVE type; prevacuole/endosomal FYVE
tethering component; class D vps; YPT5 effector; pi3p binding;
involved in golgi-vacuole transport |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1611 |
| ORF length (spliced) |
|
| Entry clone length |
1611 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1490A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC17G6.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAAAATGGTAAAAGACG |
| Rev primer name |
SPAC17G6.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATATCAAAAAGATCCAAG |
| Amino acid length |
536 |
| Molecular weight |
62.4 |
| Isoelectric point (calc.) |
7.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
Golgi; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |