| Gene name |
SPBC2G2.12 |
| Gene ID |
25/H06 |
| Gene synonyms/obsolete |
|
| Gene product |
histidine-tRNA ligase;
involved in histidyl-tRNA aminoacylation; histidine-tRNA
ligase activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1617 |
| ORF length (spliced) |
|
| Entry clone length |
1617 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1458A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC2G2.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGATTCTCTAGTAGA |
| Rev primer name |
SPBC2G2.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCGTATTTAAGTAACTTT |
| Amino acid length |
538 |
| Molecular weight |
60.2 |
| Isoelectric point (calc.) |
6.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLQLKNLKL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
| Microscope used for
observation |
DeltaVision |