| Gene name |
SPBC19C2.10 |
| Gene ID |
25/H11 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; src (SH3) homology domain; no apparent Sc
ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1621 |
| ORF length (spliced) |
1506 |
| Entry clone length |
1621 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
60T:C / 1100T:C /
1111T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC19C2.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACAAACATTTTGCAAA |
| Rev primer name |
SPBC19C2.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTAAAGCCCTTTTTCAAA |
| Amino acid length |
501 |
| Molecular weight |
56.7 |
| Isoelectric point (calc.) |
6.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
periphery at site of
septum formation; cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
| Microscope used for
observation |
DeltaVision |