Gene name |
SPCC1919.11 |
Gene ID |
25/H12 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; src (SH3) homology domain; no apparent Sc
ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1621 |
ORF length (spliced) |
1263 |
Entry clone length |
1621 |
No. of intron |
6 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1919.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTGTCTAGATTTTTTTC |
Rev primer name |
SPCC1919.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTATTCGGAATTTCAGGT |
Amino acid length |
420 |
Molecular weight |
48.3 |
Isoelectric point (calc.) |
8.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
periphery at site of
septum formation; nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |