| Gene name |
SPBC16A3.12c |
| Gene ID |
26/A11 |
| Gene synonyms/obsolete |
|
| Gene product |
triglyceride
lipase-cholesterol esterase; similar to Sp SPCC1672.09 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1632 |
| ORF length (spliced) |
1332 |
| Entry clone length |
1632 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
1418T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC16A3.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAGGATTCAATAAAAA |
| Rev primer name |
SPBC16A3.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGAAAACCGTTTTGTTTA |
| Amino acid length |
443 |
| Molecular weight |
51.5 |
| Isoelectric point (calc.) |
7.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytoplasmic dots;
vacuole? |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |