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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17G6.01
Gene ID 26/C02
Gene synonyms/obsolete SPAC17A2.14
Gene product MIT family metal ion transporter
Entry clone Cloned
ORF length (unspliced) 1854
ORF length (spliced)
Entry clone length 1854
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17G6.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCATCCAATACATCCCG
Rev primer name SPAC17G6.01.Rv
Rev primer SEQ AGAAAGCTGGGTAAATAACTTTGTACCATTTA
Amino acid length 617
Molecular weight 71.1
Isoelectric point (calc.) 5.4
Signal SEQ
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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