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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC323.03c
Gene ID 26/C09
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1865
ORF length (spliced) 1728
Entry clone length 1865
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC323.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAGAAGCCATTAACAA
Rev primer name SPAC323.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAACTGCACGACCTACGACA
Amino acid length 575
Molecular weight 66.1
Isoelectric point (calc.) 7.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LPTAVLCHLLL/LFLLTKTLNL
Localization (YFP) SPB?; dots on spindle microtubules; periphery at site of septum formation; nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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