| Gene name |
SPCC622.11 |
| Gene ID |
26/D08 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; hypothetical protein; conserved in C. elegans,
D. melanogaster CG8135 and A. thaliana; no apparent Sc
ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1884 |
| ORF length (spliced) |
1689 |
| Entry clone length |
1884 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC622.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCAGTTGGTTCAATGT |
| Rev primer name |
SPCC622.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACATGGGCATTATTTGTA |
| Amino acid length |
562 |
| Molecular weight |
64.8 |
| Isoelectric point (calc.) |
9.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
8 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIEMLGLLLLL/LYVPFVVTLLI |
| Localization (YFP) |
Golgi; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |