| Gene name |
SPCC825.02 |
| Gene ID |
26/D11 |
| Gene synonyms/obsolete |
|
| Gene product |
similar to human alpha
glucosidase II beta subunit; putative protein kinase C
substrate; EF hand (inferred from context); putative LDL
receptor |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1887 |
| ORF length (spliced) |
1521 |
| Entry clone length |
1887 |
| No. of intron |
6 |
| Sequence status |
Finished |
| Sequence results |
1397A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC825.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGTTCAGTCAATGGTA |
| Rev primer name |
SPCC825.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAACTCATCGACAGATGAT |
| Amino acid length |
506 |
| Molecular weight |
57 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTNLLDELTL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |