| Gene name |
SPBC428.17c |
| Gene ID |
26/E06 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical
serine-rich protein; sequence orphan; non-essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1894 |
| ORF length (spliced) |
1809 |
| Entry clone length |
1894 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1592A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC428.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAAGAGGAAAATGTAA |
| Rev primer name |
SPBC428.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTACCAAGTCACTGCGC |
| Amino acid length |
602 |
| Molecular weight |
67.5 |
| Isoelectric point (calc.) |
5.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFTELINLLI/LIILILGLLL |
| Localization (YFP) |
nucleus;
mitochondrion |
| Comments for localization |
faint signal of
mitochondrion |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |