Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC26H5.07c
Gene ID 26/E10
Gene synonyms/obsolete
Gene product similar to the transmembrane region of mannosyltransferases; similar to Sp SPBC18A7.02c
Entry clone Cloned
ORF length (unspliced) 1898
ORF length (spliced) 1518
Entry clone length 1898
No. of intron 2
Sequence status Finished
Sequence results 1214T:C / 1634T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC26H5.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCGATTTTAAAGTCGGC
Rev primer name SPAC26H5.07.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCAGGCTTTTGCTTAGAA
Amino acid length 505
Molecular weight 56.8
Isoelectric point (calc.) 5.3
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 8
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LITLFAMFLWI/LNNTIRDLRI/LVLILMLTI
Localization (YFP) cytoplasmic dots; Golgi?; ER
Comments for localization bright dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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