Gene name |
SPAC26H5.07c |
Gene ID |
26/E10 |
Gene synonyms/obsolete |
|
Gene product |
similar to the
transmembrane region of mannosyltransferases; similar to Sp
SPBC18A7.02c |
Entry clone |
Cloned |
ORF length (unspliced) |
1898 |
ORF length (spliced) |
1518 |
Entry clone length |
1898 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
1214T:C /
1634T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC26H5.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGATTTTAAAGTCGGC |
Rev primer name |
SPAC26H5.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCAGGCTTTTGCTTAGAA |
Amino acid length |
505 |
Molecular weight |
56.8 |
Isoelectric point (calc.) |
5.3 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
8 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LITLFAMFLWI/LNNTIRDLRI/LVLILMLTI |
Localization (YFP) |
cytoplasmic dots;
Golgi?; ER |
Comments for localization |
bright dots by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |