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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC622.20c
Gene ID 26/F03
Gene synonyms/obsolete SPCC61.01c
Gene product membrane transporter; siderochrome-iron transporter activity; involved in iron-siderochrome transport; involved in iron homeostasis; similar to Sp SPBC4F6.09 and SPAC1F8.03c
Entry clone Cloned#
ORF length (unspliced) 1906
ORF length (spliced) 1794
Entry clone length 1906
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC622.20.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTGAGAATTATGGAAG
Rev primer name SPCC622.20.Rv
Rev primer SEQ AGAAAGCTGGGTATTCTGAGGGAGTTTCGACG
Amino acid length 597
Molecular weight 66.1
Isoelectric point (calc.) 8.9
Signal SEQ
No. of transmembrane domain 12
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LGMTVLGYLSL/LGLLSQLII/LPLLASLFL/LTLFTITLSI/LSIDMSSLTL/LFMFAGLVI
Localization (YFP) vacuole membrane
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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