| Gene name |
SPCC4G3.09c |
| Gene ID |
26/F05 |
| Gene synonyms/obsolete |
|
| Gene product |
TBC domain protein;
GTPase activating protein of Rab-like GTPase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1908 |
| ORF length (spliced) |
|
| Entry clone length |
1908 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
7G:A / 18A:C / 135T:C
/ 619G:A / 1170T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC4G3.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGGCATCACTGAACA |
| Rev primer name |
SPCC4G3.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGTAGACTTCTTCAAGTGA |
| Amino acid length |
635 |
| Molecular weight |
72.7 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |