Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1105.14
Gene ID 26/F12
Gene synonyms/obsolete
Gene product zinc finger protein; zf-C2H2 type; involved in transcriptional activation
Entry clone Cloned
ORF length (unspliced) 1914
ORF length (spliced)
Entry clone length 1914
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1105.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGACACTACAAACAACAC
Rev primer name SPBC1105.14.Rv
Rev primer SEQ AGAAAGCTGGGTATCTACCGTGTTTCACACGG
Amino acid length 637
Molecular weight 69
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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