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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC736.08
Gene ID 26/G06
Gene synonyms/obsolete
Gene product DNA-binding protein; recombination signal specific; involved in meiotic recombination; no apparent Sc ortholog; non-essential
Entry clone Cloned
ORF length (unspliced) 1920
ORF length (spliced) 1842
Entry clone length 1920
No. of intron 1
Sequence status Finished
Sequence results 669T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC736.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGGGACTATTTTGCCTG
Rev primer name SPCC736.08.Rv
Rev primer SEQ AGAAAGCTGGGTAGTTTCCAAAAGCACTTGAC
Amino acid length 613
Molecular weight 67.1
Isoelectric point (calc.) 5.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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