Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1778.02
Gene ID 26/G09
Gene synonyms/obsolete rap1
Gene product involved in telomere maintenance; involved in mating-type silencing; Myb family; DNA binding protein; telomere binding is essential for meiosis; BRCT domain; interacts physically with Taz1p; recruited to the telomere by Taz1p (implicated); involved in telomeric silencing (required)
Entry clone Cloned
ORF length (unspliced) 2211
ORF length (spliced) 2082
Entry clone length 2211
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments ORF prediction was changed (extended).
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC1778.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCATTTACATTCACCAA
Rev primer name SPBC1778.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAGAAGTTTGTTTTGAAAGT
Amino acid length 693
Molecular weight 79.5
Isoelectric point (calc.) 6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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