| Gene name |
SPAC22G7.05 |
| Gene ID |
26/G11 |
| Gene synonyms/obsolete |
|
| Gene product |
involved in ribosome
biogenesis and assembly; predicted coiled-coil protein;
interacts physically with Mis3p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1924 |
| ORF length (spliced) |
1797 |
| Entry clone length |
1924 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
319A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC22G7.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCCAGAAAAAAATCAGC |
| Rev primer name |
SPAC22G7.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTCCCAAAAACTTGTTTC |
| Amino acid length |
598 |
| Molecular weight |
69.5 |
| Isoelectric point (calc.) |
4.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB?; nucleus |
| Comments for localization |
one nucleolar dot by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |