Gene name |
SPAC14C4.07 |
Gene ID |
26/H04 |
Gene synonyms/obsolete |
|
Gene product |
transporter; unknown
specificity |
Entry clone |
Cloned |
ORF length (unspliced) |
1935 |
ORF length (spliced) |
|
Entry clone length |
1935 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1427C:T /
1475T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC14C4.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTCACACGATGATGA |
Rev primer name |
SPAC14C4.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACGTTTGCGATTTAAAGGT |
Amino acid length |
644 |
Molecular weight |
71.7 |
Isoelectric point (calc.) |
7.6 |
Signal SEQ |
|
No. of transmembrane domain |
12 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSPLLILPL/LFITVSFLLL/LLAAILISLTL |
Localization (YFP) |
Golgi?; vacuole
membrane |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |