| Gene name |
SPBC1604.19c |
| Gene ID |
27/C01 |
| Gene synonyms/obsolete |
|
| Gene product |
TRAPP; involved in
intracellular protein transport; similar to Sp SPBC1734.07C
(paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1977 |
| ORF length (spliced) |
|
| Entry clone length |
1977 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1925A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1604.19.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACATTGCAACAAATTT |
| Rev primer name |
SPBC1604.19.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGAAAAGTATTTTTCACC |
| Amino acid length |
658 |
| Molecular weight |
76 |
| Isoelectric point (calc.) |
7.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNPILDYLKL |
| Localization (YFP) |
cytosol=nucleus;
periphery at site of septum formation |
| Comments for localization |
except nucleolus |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|