Gene name |
SPAC22A12.15c |
Gene ID |
27/C11 |
Gene synonyms/obsolete |
bip1; bip |
Gene product |
resident luminal ER
protein; essential; heat shock protein 70 family; involved in
assembly of multimeric complexes in the ER; interacts
physically with Cnx1p |
Entry clone |
Cloned |
ORF length (unspliced) |
1992 |
ORF length (spliced) |
|
Entry clone length |
1992 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
12C:G / 1235A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC22A12.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGAAGTTCCAGCTATT |
Rev primer name |
SPAC22A12.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGTTCATCGGCCTCATCA |
Amino acid length |
663 |
Molecular weight |
73.2 |
Isoelectric point (calc.) |
4.5 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSYFVALFL/LLDVIPLTLGI |
Localization (YFP) |
ER; Golgi |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |