Gene name |
SPBC23G7.13c |
Gene ID |
27/D03 |
Gene synonyms/obsolete |
|
Gene product |
urea transporter |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
1995 |
ORF length (spliced) |
|
Entry clone length |
1995 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC23G7.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTCCAACCCGAATTGAC |
Rev primer name |
SPBC23G7.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGACACTTTCAATGTCTTTT |
Amino acid length |
664 |
Molecular weight |
71.7 |
Isoelectric point (calc.) |
8.2 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
15 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGLGFAALMI/LSPALYILIL |
Localization (YFP) |
SPB?; nuclear
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |