| Gene name |
SPAC30D11.09 |
| Gene ID |
27/D08 |
| Gene synonyms/obsolete |
cwf19 |
| Gene product |
eukaryotic conserved
protein; zinc finger protein; involved in RNA processing |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2007 |
| ORF length (spliced) |
1920 |
| Entry clone length |
2007 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1060C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC30D11.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAAAGGAAAAGCTTAC |
| Rev primer name |
SPAC30D11.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCAATTAATCCCTTGGTC |
| Amino acid length |
639 |
| Molecular weight |
74.4 |
| Isoelectric point (calc.) |
7.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Confocal
|