Gene name |
SPAC23A1.01c |
Gene ID |
27/E01 |
Gene synonyms/obsolete |
SPAC19G12.16c |
Gene product |
Sp specific;
serine/threonine-rich protein; glycoprotein; similar to alpha
agglutinins; localization cell surface; GPI anchored
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
2013 |
ORF length (spliced) |
|
Entry clone length |
2013 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC23A1.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGAAGATTAACCATCAG |
Rev primer name |
SPAC23A1.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGAATAAAAACAATAAAGGG |
Amino acid length |
670 |
Molecular weight |
68.2 |
Isoelectric point (calc.) |
4.2 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLCVFIPLLFL |
Localization (YFP) |
ER, only? |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|