Gene name |
SPAC4G8.07c |
Gene ID |
27/E04 |
Gene synonyms/obsolete |
|
Gene product |
uridine
methyltransferase; catalyzes the formation of ribothymidine in
tRNAs |
Entry clone |
Cloned |
ORF length (unspliced) |
2014 |
ORF length (spliced) |
1584 |
Entry clone length |
2014 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
1087T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC4G8.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTATCCATATTGTTTGAA |
Rev primer name |
SPAC4G8.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGATACAACCTTTGTCAAT |
Amino acid length |
527 |
Molecular weight |
59.6 |
Isoelectric point (calc.) |
8.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus;
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|