| Gene name |
SPAC1687.20c |
| Gene ID |
27/E07 |
| Gene synonyms/obsolete |
mis6 |
| Gene product |
inner centromere
protein; involved in chromosome segregation, sister chromatid
segregation (G1/S phase) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2019 |
| ORF length (spliced) |
|
| Entry clone length |
2019 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
472A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1687.20.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAAGCTTTGAGAATAA |
| Rev primer name |
SPAC1687.20.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACGATTTAATGTTGAAAGT |
| Amino acid length |
672 |
| Molecular weight |
78.5 |
| Isoelectric point (calc.) |
9.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFHFLDFLTL/LHITLNYGLAL/LWLWLFRMLNL |
| Localization (YFP) |
SPB; cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |