| Gene name |
SPBC25H2.16c |
| Gene ID |
27/F01 |
| Gene synonyms/obsolete |
|
| Gene product |
VHS domain; GAT
domain; involved in intracellular protein transport; adaptin
C-terminal domain; similar to S. pombe SPAC1F3.05 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2041 |
| ORF length (spliced) |
1602 |
| Entry clone length |
2041 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC25H2.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAGCGCTAAAAGAAG |
| Rev primer name |
SPBC25H2.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGAGTAGCCTAATTGAAGAT |
| Amino acid length |
533 |
| Molecular weight |
59.4 |
| Isoelectric point (calc.) |
5.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
Golgi |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |