| Gene name |
SPBC1703.06 |
| Gene ID |
27/F04 |
| Gene synonyms/obsolete |
pof10 |
| Gene product |
F-box protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2043 |
| ORF length (spliced) |
1989 |
| Entry clone length |
2043 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1703.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAATCGGAACCAACATC |
| Rev primer name |
SPBC1703.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACATTTCCATGAGTGATAAT |
| Amino acid length |
662 |
| Molecular weight |
74.2 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
| Microscope used for
observation |
Confocal
|