Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC947.02
Gene ID 27/F07
Gene synonyms/obsolete apl2
Gene product AP-1 adaptor complex subunit; adaptin family; involved in vesicle-mediated transport
Entry clone Cloned in 2006 trial
ORF length (unspliced) 2052
ORF length (spliced)
Entry clone length 2052
No. of intron 0
Sequence status Partially sequenced
Sequence results 100% match in both ends
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC947.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTTCCAAAGTTGTTTCA
Rev primer name SPBC947.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGCCCTAACAGATCATCT
Amino acid length 683
Molecular weight 76.6
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKKLVYLYL/LYIKMEKLKI/LLTAVVKLSL
Localization (YFP) no expression clone
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation

Image information
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