Gene name |
SPBC4F6.07c |
Gene ID |
27/G01 |
Gene synonyms/obsolete |
|
Gene product |
DEAD/DEAH box
helicase; ATP-dependent |
Entry clone |
Cloned |
ORF length (unspliced) |
2071 |
ORF length (spliced) |
1947 |
Entry clone length |
2071 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
326T:C / 417T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC4F6.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCAAAGAAAGCTGCATT |
Rev primer name |
SPBC4F6.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTTCTTCCTTTTTTGCAGA |
Amino acid length |
648 |
Molecular weight |
73.2 |
Isoelectric point (calc.) |
9.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
65 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal; DeltaVision
|