Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC18B11.10
Gene ID 27/G06
Gene synonyms/obsolete tup11
Gene product transcriptional repressor; histone binding; glucose repression regulatory protein; WD repeat protein
Entry clone Cloned
ORF length (unspliced) 2084
ORF length (spliced) 1854
Entry clone length 2084
No. of intron 2
Sequence status Finished
Sequence results 404A:deletion / 1372G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC18B11.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCGTCAGTGGAGGATGC
Rev primer name SPAC18B11.10.Rv
Rev primer SEQ AGAAAGCTGGGTAAGGAGATGCAGGGTCAATT
Amino acid length 614
Molecular weight 67.3
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.