Gene name |
SPBC418.02 |
Gene ID |
27/G10 |
Gene synonyms/obsolete |
|
Gene product |
N-acetyltransferase
complex (putative); TPR repeat protein; similar to Sp
SPCC338.07C |
Entry clone |
Cloned |
ORF length (unspliced) |
2088 |
ORF length (spliced) |
|
Entry clone length |
2088 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC418.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTAAATTGTCCGAAAA |
Rev primer name |
SPBC418.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAATACTGAAGTTTCTAGA |
Amino acid length |
695 |
Molecular weight |
81 |
Isoelectric point (calc.) |
7.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRQKLYGLAL/LRATLGALTI |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|