Gene name |
SPAC6G9.14 |
Gene ID |
27/H03 |
Gene synonyms/obsolete |
|
Gene product |
serine-rich protein;
pumilio family; RNA-binding protein; involved in the transport
of telomere silencing proteins |
Entry clone |
Cloned |
ORF length (unspliced) |
2096 |
ORF length (spliced) |
2046 |
Entry clone length |
2096 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
190A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC6G9.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACCGAGCAGTCTTCTAA |
Rev primer name |
SPAC6G9.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCTGAAGAGGAAGTATTA |
Amino acid length |
681 |
Molecular weight |
73.2 |
Isoelectric point (calc.) |
8.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSSSVASLSL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|